Pet infusion is often performed when there is severe blood loss, severe anemia, increased blood solidity, or to improve the pet's endurance. Although there are blood types in pets, blood types are often not considered when giving pets their first blood transfusion.

(1) Selection of blood donor animals

Choose pets of the same species that are strong, healthy (clinical and laboratory tests) and not too obese. A suitable weight for dogs is around 20kg, and for cats, a weight of around 2kg is suitable.

(2) Blood type and blood compatibility test

Blood type and cross-match tests are suitable for repeated blood transfusions of the same pet. Dogs have 7 blood factors, of which blood type A factor is the most important. Transfusing A-positive blood into A-negative dogs can promote the production of anti-A antibodies. The antibody has strong hemolytic activity in the test tube and in the body. If a male dog stimulated by A-positive blood mates with an A-positive male dog, the offspring will develop neonatal hemolytic disease after breastfeeding. Among the dogs, 37% of the dogs are A negative and 63% of the dogs are A positive. If blood transfusion is blind, 25% of the dogs can produce anti-A antibodies. In the second blood transfusion, 15% of blindly selected blood donor dogs will be unsuitable blood transfusions, and it is very dangerous to transfuse A-positive blood to A-negative dogs 2-3 times. At present, it is not possible to determine the blood type of a dog through accurate measurement, so only a blood compatibility test is used to determine whether the two are suitable. The specific methods are as follows:

① Collect anticoagulated blood from donors and recipients, and collect serum and red blood cells (anticoagulated and non-anticoagulated) respectively.

② Wash the red blood cells three times and use plasma to make a 5%-10% red blood cell suspension.

③ Add 2 drops of serum from the recipient individual and red blood cell suspension from the donor individual into a thin test tube (diameter 7mm), and mix. Then add 2 drops of serum from the donor and 2 drops of red blood cells from the recipient to another test tube and mix.

④ Place the test tube at room temperature for 30 minutes, then centrifuge at 1000 rpm for 1 minute.

⑤ If there is obvious hemolysis or coagulation in any combination, it means that the blood of the donor and the recipient is not consistent.

⑥It is simpler to mix the serum and plasma of the donor and recipient on a glass slide, and hemolysis and coagulation will cause blood inconsistency.

⑦ You can also use the three-drop test method: take a clean glass slide, drop a drop of normal saline in the center, drop a drop of donor blood and recipient blood on both sides of the normal saline drop, and use a glass rod to evenly distribute the three drops. Mix, let it sit for 3-5 minutes, observe the state of the mixture, if the mixture is evenly distributed, blood can be provided. If particles precipitate out of the mixture, it means that the blood types of the two are incompatible and they cannot be used as blood donors.