It can be used when dogs are bleeding. Counting from the first time you see blood dripping from the vulva of a bitch in estrus, it is generally more appropriate to breed a primiparous bitch on the 11th to 13th day, and for a multiparous bitch on the 9th to 11th day. It is recommended to mate more times to increase the success rate.

The best breeding period for most female dogs is 9-13 days after bleeding from the female dog’s vagina. However, there are also a very small number of multiparous bitches whose vaginas stop bleeding within 5-6 days of being in estrus after multiple births. They can also be bred to get pregnant within the next 1-2 days.