1. Commonly used inspection methods

【1. Clinical examination method】

1.Skin scraping examination

Skin scraping is one of the most commonly used skin disease detection methods in veterinary clinical practice. This method is simple and fast, and can identify many types of parasitic infections. Although skin scraping does not provide diagnostic evidence every time, its simplicity and low price make it one of the most common methods for detecting skin diseases.

Some clinicians like to reuse blunt blades for skin scraping. However, with the increasing awareness of prevention of infectious diseases (Bartonella, feline leukemia, feline AIDS, herpes virus, papilloma virus, etc.), This phenomenon will be gradually reduced.

Skin scrapings are divided into superficial skin scrapings and deep skin scrapings

Superficial skin scrapings are used for the observation of scabies, chelicerae, ear mites and chiggers

Deep skin scrapings are mainly used for the diagnosis of demodicosis

2. Cytological examination

Skin cytology is the second most commonly used skin disease detection technology, mainly used for the identification of bacteria, fungi, infiltrating cells, tumor cells and spiny cells.

3.Scotch tape method:

Can be used to detect a variety of skin diseases.

4. Ear test examination:

It can be used to check whether there are exudative lesions inside the normal-appearing ear canal, and to identify whether there are parasites, fungi, and bacterial infections in the external auditory canal.

5. Hair plucking inspection:

Hair plucking examination is used to observe the condition of hair, and can complete the assessment of the body's itching status, fungal infection, pigmentation, growth cycle, etc.

6. Wood's lamp examination:

The light source used by Wood's lamp is special ultraviolet light with a wavelength of 340-450nm. The ultraviolet light in this band will not damage the skin or eyes, but can make the tryptophan metabolites produced by the metabolism of some microorganisms such as microspores emit apple-green fluorescence. However, not all microspores produce this cellular metabolite, and only about 50% of canine microspores can be detected by Wood's lamp method. Trichophyton and Microsporum gypseum are not detectable by Wood's lamp examination.

7. Slide pressure diagnosis method

Mainly used to identify vasodilation and subcutaneous hematoma.

【2. Special inspection method】

1.Cultivation method:

​Used to identify specific pathogenic microorganisms

DTM (Fungi Detection Medium) is used for the isolation and identification of dermatophytes.The dermatophyte test medium is composed of special material that inhibits the growth of bacteria, and the medium turns red when fungi are present.

Bacterial/fungal culture plays an important role in the diagnosis of skin diseases. Any deep cellulitis, especially cellulitis that has formed a leak, should be cultured for bacteria/fungi. When making a differential diagnosis, cysts and tumors should also be cultured. Culture and testing for pathogenic microorganisms should be performed.

2. Biopsy:

For veterinary workers, biopsy is a very troublesome detection technology. The correct selection of the lesion site is of great significance to improving the reliability of skin biopsy results.

Skin biopsies provide the most useful information in a very short period of time. Although skin histopathology cannot correctly identify the cause, they can characterize skin lesions into 1-6 general categories: neoplastic, infectious Sexual, immune-mediated, endocrine-like, keratinization defects, allergies.

3.PCR technology:

Polymerase chain reaction can amplify DNA in sample crystals. Compared with other detection technologies, PCR technology has higher sensitivity and specificity.

4. Immunohistochemistry:

Irreplaceable method for diagnosing autoimmune skin diseases

5. Allergy test:

Used in the following situations: 1. Animals with clinical symptoms of allergic skin disease. 2. Animals whose biopsies/skin scrapings indicate allergic status

6. Therapeutic Diagnosis

Determine skin allergens or infectious agents.